期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 24, 期 6, 页码 3256-3270出版社
WILEY
DOI: 10.1111/jcmm.15039
关键词
base editor; CRISPR imaging; CRISPR-based genome-wide screening; CRISPR-Cas systems; gene edting; genome editing; RNA editing
资金
- National Natural Science Foundation of China [31771635]
- Research Grants Council, University Grants Committee [14119518, T13-605/18-W, T13-402/17-N]
- Children's Thalassaemia Foundation in Hong Kong [CTFHK 2018/02]
The CRISPR-Cas technologies derived from bacterial and archaeal adaptive immune systems have emerged as a series of groundbreaking nucleic acid-guided gene editing tools, ultimately standing out among several engineered nucleases because of their high efficiency, sequence-specific targeting, ease of programming and versatility. Facilitated by the advancement across multiple disciplines such as bioinformatics, structural biology and high-throughput sequencing, the discoveries and engineering of various innovative CRISPR-Cas systems are rapidly expanding the CRISPR toolbox. This is revolutionizing not only genome editing but also various other types of nucleic acid-guided manipulations such as transcriptional control and genomic imaging. Meanwhile, the adaptation of various CRISPR strategies in multiple settings has realized numerous previously non-existing applications, ranging from the introduction of sophisticated approaches in basic research to impactful agricultural and therapeutic applications. Here, we summarize the recent advances of CRISPR technologies and strategies, as well as their impactful applications.
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