期刊
JOURNAL OF BIOPHOTONICS
卷 13, 期 6, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201960243
关键词
3D imaging; add-on device; light-sheet microscopy; open source; voxel super-resolution
资金
- Junior Thousand Talents Program of China
- Fundamental Research Funds for the Central Universities [2019JYCXJJ012, 2019kfyRCPY060]
- Wuhan Morning Light Plan of Youth Science and Technology [2017050304010295]
- National Science Foundation of Hubei [2018CFA039]
- National Natural Science Foundation of China [31671052, 31871069, 61860206009, 21874052]
- Research Program of Shenzhen [JCYJ20160429182424047]
- National Key RAMP
- D program of China [2017YFA0700501]
- Innovation Fund of WNLO
Light-sheet fluorescence microscopy (LSFM) allows volumetric live imaging at high-speed and with low photo-toxicity. Various LSFM modalities are commercially available, but their size and cost limit their access by the research community. A new method, termed sub-voxel-resolving (SVR) light-sheet add-on microscopy (SLAM), is presented to enable fast, resolution-enhanced light-sheet fluorescence imaging from a conventional wide-field microscope. This method contains two components: a miniature add-on device to regular wide-field microscopes, which contains a horizontal laser light-sheet illumination path to confine fluorophore excitation at the vicinity of the focal plane for optical sectioning; an off-axis scanning strategy and a SVR algorithm that utilizes sub-voxel spatial shifts to reconstruct the image volume that results in a twofold increase in resolution. SLAM method has been applied to observe the muscle activity change of crawling C. elegans, the heartbeat of developing zebrafish embryo, and the neural anatomy of cleared mouse brains, at high spatiotemporal resolution. It provides an efficient and cost-effective solution to convert the vast number of in-service microscopes for fast 3D live imaging with voxel-super-resolved capability.
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