期刊
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
卷 39, 期 5, 页码 1561-1571出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2020.1734093
关键词
3-amido-9-ethylcarbazole; calf thymus DNA; groove binding; molecular docking; FRET
资金
- Hacettepe University Scientific Research Unit [12854]
The new CMR compound showed static binding to DNA and HSA with hydrophobic interaction playing a predominant role. Competition assays revealed CMR primarily binds in the A-T region of DNA's minor groove.
In this work, we presented the synthesis and investigation of binding properties of the new morpholine containing 3-amido-9-ethylcarbazole derivative (CMR) to calf thymus DNA (ctDNA) and human serum albumin (HSA) by fluorescence spectroscopy, UV absorption spectroscopy and molecular docking method. A decrease in Stern-Volmer constants was obtained with increase in temperature; it shows that static quenching mechanism leads to formation of new CMR-DNA/HSA complexes, which have hydrophobic interaction as the predominant role in the binding modes. Also, binding properties of DNA were investigated with competition assays on two probes (EB and H33258) by absorption, ionic strength and iodide ion quenching methods. The results suggested that CMR entered into the minor groove binding on the A-T region of DNA. The spectral data further confirmed by molecular docking which elicited that CMR complexes have similar interaction and conformation trends to each target, DNA and HSA. The experimental and computational results show that CMR has been classified as a promising molecule in drug designing of other carbazole derivatives. Communicated by Ramaswamy H. Sarma
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