期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 295, 期 15, 页码 4985-5001出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA120.012546
关键词
prion; genetic polymorphism; conformational change; oligomer; prion disease; host?pathogen interaction; evolution; host range; strains
资金
- Alberta Prion Research Institute (APRI) [PEX15023]
- Genome Canada
- Genome Alberta
- APRI
- University of Alberta
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2017-05539]
- Alberta Innovates Biosolutions [FP00204477]
- National Institutes of Health [1R01NS103848]
Chronic wasting disease (CWD) is caused by an unknown spectrum of prions and has become enzootic in populations of cervid species that express cellular prion protein (PrP (c)) molecules varying in amino acid composition. These PrP (c) polymorphisms can affect prion transmission, disease progression, neuropathology, and emergence of new prion strains, but the mechanistic steps in prion evolution are not understood. Here, using conformation-dependent immunoassay, conformation stability assay, and protein-misfolding cyclic amplification, we monitored the conformational and phenotypic characteristics of CWD prions passaged through deer and transgenic mice expressing different cervid PrP (c) polymorphisms. We observed that transmission through hosts with distinct PrP (c) sequences diversifies the PrPCWD conformations and causes a shift toward oligomers with defined structural organization, replication rate, and host range. When passaged in host environments that restrict prion replication, distinct co-existing PrPCWD conformers underwent competitive selection, stabilizing a new prion strain. Nonadaptive conformers exhibited unstable replication and accumulated only to low levels. These results suggest a continuously evolving diversity of CWD conformers and imply a critical interplay between CWD prion plasticity and PrP (c) polymorphisms during prion strain evolution.
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