期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 295, 期 11, 页码 3719-3733出版社
ELSEVIER
DOI: 10.1074/jbc.RA119.010910
关键词
cell migration; cell adhesion; glycoprotein structure; kinetics; glycobiology; carbohydrate-binding protein; dimerization; binding kinetics; complement regulatory repeats; E-selectin; short consensus repeats; silkworm expression; adhesion; glycoprotein; conformational extension; homing; silkworm expression
资金
- King Abdullah University of Science and Technology (KAUST)
- [OCRF-2014-CRG3-2276]
Selectins are key to mediating interactions involved in cellular adhesion and migration, underlying processes such as immune responses, metastasis, and transplantation. Selectins are composed of a lectin domain, an epidermal growth factor (EGF)-like domain, multiple short consensus repeats (SCRs), a transmembrane domain, and a cytoplasmic tail. It is well-established that the lectin and EGF domains are required to mediate interactions with ligands; however, the contributions of the other domains in mediating these interactions remain obscure. Using various E-selectin constructs produced in a newly developed silkworm-based expression system and several assays performed under both static and physiological flow conditions, including flow cytometry, glycan array analysis, surface plasmon resonance, and cell-rolling assays, we show here that a reduction in the number of SCR domains is correlated with a decline in functional E-selectin binding to hematopoietic cell E- and/or L-selectin ligand (HCELL) and P-selectin glycoprotein ligand-1 (PSGL-1). Moreover, the binding was significantly improved through E-selectin dimerization and by a substitution (A28H) that mimics an extended conformation of the lectin and EGF domains. Analyses of the association and dissociation rates indicated that the SCR domains, conformational extension, and dimerization collectively contribute to the association rate of E-selectin?ligand binding, whereas just the lectin and EGF domains contribute to the dissociation rate. These findings provide the first evidence of the critical role of the association rate in functional E-selectin?ligand interactions, and they highlight that the SCR domains have an important role that goes beyond the structural extension of the lectin and EGF domains.
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