期刊
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
卷 104, 期 2, 页码 735-748出版社
WILEY
DOI: 10.1111/jpn.13290
关键词
characterization; Flammulina velutipes; gut microbiota; immunomodulatory; polysaccharide
资金
- Guangdong Natural Science Foundation [C1051164]
- Guangdong Regular Institutions of Higher Learning Characteristic Innovation Projects [2018KTSCX030]
- High-level Talent Introduction Project [C1034220]
- National Natural Science Foundation of China [81973013]
The structural characteristics of a novel Flammulina velutipes polysaccharide (FVP2) were explored in this study. Besides, immunomodulatory activities of FVP2 on RAW 264.7 cell and its impact on gut microbiota in rats were investigated. FVP2 has a molecular weight of 18.3 kD, and its main components include galactose (19.96%), glucose (60.66%) and mannose (19.38%). By NMR analysis, the main-chain structure consisted of (1 -> 3)-linked-beta-D-Gal, (1 -> 6) -linked-beta-D-Gal, (1 -> 6)-linked-alpha-D-Glc and (1 -> 3,6)-linked-alpha-D-Man was identified. Results of the in vitro assays on RAW 264.7 murine macrophage cells showed FVP2 could significantly up-regulate the expression of NO, TNF-alpha and IL-6. FVP2 was intragastrically administered to rats for 2 weeks. Compared with the control group, two caecal short-chain fatty acids (SCFAs) concentration (isobutyric acid and butyric acid) and the abundance of beneficial microbiota of the FVP2-treated group were significantly increased (p < .05) respectively. The results demonstrated that FVP2 could effectively enhance the level of butyric acid and increase beneficial gut microbiota, which could improve the intestinal barrier function and maintain the intestinal mucosal integrity, suggesting that FVP2 could potentially be an immunomodulators or a functional food to promote intestinal health.
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