期刊
BRITISH JOURNAL OF NUTRITION
卷 116, 期 6, 页码 1116-1124出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114516002816
关键词
Okara; Hydrostatic pressure; Ultraflo ((R)) L enzyme; Fermentation; Microbiota; Prebiotics; Food by-products
资金
- Education, Language Policy and Culture of the Basque Government (Spain) [PRE_2013_1_682]
- Nutrition of ICTAN-CSIC in Madrid
- Food & Nutritional Sciences Unit, School of Chemistry, Food and Pharmacy, University of Reading, UK
At present, there is a huge interest in finding new prebiotics from agrofood industrial waste, such as the soyabean by-product Okara, rich in insoluble dietary fibre. A previous treatment of Okara with high hydrostatic pressure assisted by the food-grade enzyme Ultraflo((R)) L achieved a 582 % increment in its soluble dietary fibre (SDF) contents. Therefore, potential prebiotic effect of both treated and native Okara was assayed using 48 h, pH-controlled, anaerobic batch cultures inoculated with human faecal slurries, which simulate the human gut. Changes in faecal microbiota were evaluated using 16S rRNA-based fluorescence in situ hybridisation, whereas release of SCFA and lactic acid was assessed by HPLC. Both Okara samples exhibited potential prebiotic effects but Okara treated to maximise its SDF content showed higher SCFA plus lactic acid, better growth promotion of beneficial bacteria, including bifidobacteria after 4 and 48 h and lactobacilli after 4 h of fermentation, and a greater inhibition of potentially harmful bacterial groups such as clostridia and Bacteroides. Differences found between fructo-oligosaccharides and Okara substrates could be attributed to the great complexity of Okara's cell wall, which would need longer times to be fermented than other easily digested molecules, thus allowing an extended potential prebiotic effect. These results support an in vitro potential prebiotic effect of Okara.
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