4.7 Article

Methyl Jasmonate Enhances Ethylene Synthesis in Kiwifruit by Inducing NAC Genes That Activate ACS1

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 10, 页码 3267-3276

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b07379

关键词

NAC; ethylene; methyl jasmonate; ACS; kiwifruit

资金

  1. National Key Research and Development Program [2018YFD1000200]
  2. National Natural Science Foundation of China [31722042]
  3. Natural Science Foundation of Zhejiang Province, China [LR16C150001]
  4. Fok Ying Tung Education Foundation, China [161028]

向作者/读者索取更多资源

Cross-talk between various hormones is important in regulating many aspects of plant growth, development, and senescence, including fruit ripening. Here, exogenous ethylene (ETH, 100 mu L/L, 12 h) rapidly accelerated 'Hayward' kiwifruit (Actinidia deliciosa) softening and ethylene production and was enhanced by supplementing with continuous treatment with methyl jasmonate (MeJA, 100 mu M/L, 12 h) (ETH+ MeJA). ETH+MeJA enhanced ACC synthase (ACS) activities and 1-aminocyclopropane-1-carboxylic acid (ACC) accumulation but not ACC oxidase (ACO) activity. Increased transcripts of ACS genes AdACS1 and AdACS2, ACS activity, and ethylene production were positively correlated. The abundance of AdACS1 was about 6-fold higher than AdACS2. RNA-seq identified 6 transcription factors among the 87 differentially expressed unigenes induced by ETH+MeJA. Dual-luciferase and electrophoretic mobility shift assays (EMSA) indicated that AdNAC2/3 physically interacted with and trans-activated the AdACS1 promoter 2.2- and 3.5-fold, respectively. Collectively, our results indicate that MeJA accelerates ethylene production in kiwifruit induced by exogenous ethylene, via a preferential activation of AdACS1 and AdACS2.

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