期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 1, 页码 376-383出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b06223
关键词
zearalenone; ZEN-degrading lactonase; thermostability; modification
资金
- Natural Science Foundation of China [31922073, 31801583]
- Natural Science Foundation of Jiangsu Province [BK20180607]
The occurrence of mycotoxin zearalenone (ZEN) and its derivatives has been a severe global threat to food and animals. In addition to the chemical and physical degradation methods, a powerful biocatalyst is urgently required for the detoxification of ZEN. Here, an efficient ZEN-degrading lactonase from Gliocladium roseum, named ZENG, was identified for the first time. The recombinant ZENG exhibited the highest activity at pH 7.0 and 38 degrees C. In addition, the recombinant enzyme showed a high degrading performance toward ZEN and its toxic derivatives alpha-zearalenol (alpha-ZOL) and alpha-zearalanol (alpha-ZAL), with the specific activities as 315, 187, and 117 units/mg, respectively. To meet the industrial demands, attempts were also made to enhance the thermostability of ZENG using a structure-based modification. Three double-site mutants, including H134L/S136L, H134F/S136F, and H134I/S134I, in the position between the cap and core catalytic domain of ZENG were designed. Finally, the thermostability of both H134L/S136L and H134F/S136F displayed a significant improvement compared to the wild-type enzyme.
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