期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 4, 页码 1091-1100出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b06094
关键词
pulse cotyledon cells; cell wall intactness; fermentation rate; short-chain fatty acids; gut microbiota
资金
- National Natural Science Foundation of China [31701546]
- Natural Science Foundation of Guangdong Province [2017A030313207]
- Fundamental Research Funds for the Central Universities of China [2019ZD40, 2018KZ13]
- 111 Project [B17018]
- Hong Kong Scholar Program [XJ2019049]
- Pearl River Talent Recruitment Program of Guangdong Province [2017GC010229]
- Pearl River Nova Program of Guangzhou [201906010079]
The physical structure of type 1 resistant starch (RS 1) could influence the metabolite production and stimulate the growth of specific bacteria in the human colon. In the present study, we isolated intact cotyledon cells from pinto bean seeds as whole pulse food and RS 1 model and obtained a series of cell wall integrities through controlled enzymolysis. In vitro human fecal fermentation performance and microbiota responses were tested, and we reported that the cell wall integrity controls the in vitro fecal fermentation rate of heat-treated pinto bean cells. The concentration of butyrate produced by pinto bean cell fermentation enhanced with weakened cell wall integrity, and certain beneficial bacterial groups such as Blautia and Roseburia genera were remarkably promoted by pinto bean cells with damaged cell wall integrity. However, the intact cell sample had a shape more similar to microbiota composition with the purified cell wall polysaccharides, rather than the damaged cells.
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