Pararobbsia silviterrae gen. nov., sp. nov., isolated from forest soil and reclassification of Burkholderia alpina as Pararobbsia alpina comb. nov.

A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain. DHC34(T). was isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, China (112 degrees 31' E 23 degrees 10' N). It grew optimally on R2A medium at 28 degrees C, at pH 6.0-7.0 and in the presence of 0-1% (w/v) NaCl. Strain DHC34(T) was closely related to Burkholderia alpina LMG 28138(T) (98.5% 16S rRNA gene sequence similarity). 16S rRNA gene sequence analysis showed that strain DHC34(T) formed a Glade with B. alpina LMG 28138(T). which is next to but branched deeply with Robbsia andropogonis ICMP 2807(T). The phylogenetic relationships among these three strains were also supported with the phylogram based on concatenated partial gyrB, recA and trpB gene sequences. The phylogenomic tree generated with the UBCG tool showed that strains DHC34(T) and R. andropogonis ICMP 2807(T) were in a different Glade. The DNA-DNA relatedness values between strain DHC34(T) and B. alpina LMG 28138(T) and R. andropogonis ICMP 2807(T) were much lower than 70%. Strain DHC34(T) contained ubiquinone 8 as the major respiratory quinone. Its major fatty acids were C16:0, C-17:0 cyclo and C-19:0 cyclo omega 8c. The DNA G+C content of strain DHC34(T) was 64.2 mol%. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, three unidentified aminophospholipids, four unidentified phospholipids, one unidentified aminolipid and a polar lipid. The phenotypic, phylogenetic, genotypic and chemotaxonomic data showed that strain DHC34(T) represents a novel species of a new genus in the family Burkholderiaceae, for which the name Pararobbsia silviterrae gen. nov., sp. nov. is proposed. The type strain of Pararobbsia silviterrae is DHC34(T) (=KCTC 42628(T)=LMG 28845(T)). On the basis of the current data, Burkholderia alpina is renamed as Pararobbsia alpina comb. nov.