Pseudomonas haemolytica sp. nov., isolated from raw milk and skimmed milk concentrate

Two strains, WS 5063(T) and WS 5067, isolated from raw cow's milk and skimmed milk concentrate, could be affiliated as members of the same, hitherto unknown, Pseudomonas species by 16S rRNA and rpoD gene sequences. Multilocus sequence and average nucleotide identity (ANIm) analyses based on draft genome sequences confirmed the discovery of a novel Pseudomonas species. It was most closely related to Pseudomonas synxantha DSM 18928(T) with an ANIm of 91.4%. The DNA G+C content of WS 5063 1 was 60.0 mol%. Phenotypic characterizations showed that the isolates are rod-shaped, motile, catalaseand oxidase-positive, and aerobic. Growth occurred at 4-34 degrees C and at pH values of pH 5.5-8.0. Both strains showed strong beta-haemolysis on blood agar. The major cellular polar lipids were phosphatidylethanolamine. phosphatidylglycerol and diphosphatidylglycerol. The dominant quinone was Q-9 (90%), but noticeable amounts of Q-8 (9%) and traces of Q-7 were also detected. Fatty acid profiles were typical for Pseudomonas species and exhibited C-6:0 as a major component. Based on these results, we conclude that both strains belong to a novel species, for which the name Pseudomonas haemolytica sp. nov. is proposed. The type strain is WS 5063(T) (=DSM 108987(T) =LMG 31232(T)) and an additional strain is WS 5067 (=DSM 108988=LMG 31233).