期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 20, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/ijms20246234
关键词
hippocampus; hyperhomocysteinemia; neurodegeneration; methionine diet; 1H MRS
资金
- Ministry of Education of the Slovak Republic [VEGA 1/0171/18, VEGA 1/0128/16, APVV 15/0107]
- Ministry of Health of the Slovak Republic
- project Identification of novel markers in diagnostic panel of neurological diseases [26220220114]
- EU
- European Regional Development Fund
Hyperhomocysteinemia (hHcy) is regarded as an independent and strong risk factor for cerebrovascular diseases, stroke, and dementias. The hippocampus has a crucial role in spatial navigation and memory processes and is being constantly studied for neurodegenerative disorders. We used a moderate methionine (Met) diet at a dose of 2 g/kg of animal weight/day in duration of four weeks to induce mild hHcy in adult male Wistar rats. A novel approach has been used to explore the hippocampal metabolic changes using proton magnetic resonance spectroscopy (1H MRS), involving a 7T MR scanner in combination with histochemical and immunofluorescence analysis. We found alterations in the metabolic profile, as well as remarkable histo-morphological changes such as an increase of hippocampal volume, alterations in number and morphology of astrocytes, neurons, and their processes in the selective vulnerable brain area of animals treated with a Met-enriched diet. Results of both methodologies suggest that the mild hHcy induced by Met-enriched diet alters volume, histo-morphological pattern, and metabolic profile of hippocampal brain area, which might eventually endorse the neurodegenerative processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据