期刊
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷 55, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijantimicag.2020.105892
关键词
Elansolid-type of polyketide spanned macrolides; Bacillus amyloliquefaciens MTCC 12716; Drug-resistant bacteria; Natural product gene clusters; Methicillin-resistant Staphylococcus aureus; Peptide deformylase
资金
- Kerala State Council for Science, Technology and Environment [040/FSHP-LSS/2014/KSCSTE]
- Science and Engineering Research Board (SERB) Scheme [SR/S1/OC-96A/2012]
- Department of Science and Technology (New Delhi, India)
Three homologous oxygenated elansolid-type of polyketide spanned macrolides were isolated from a heterotrophic marine bacterium, Bacillus amyloliquefaciens MTCC 12716, associated with an intertidal red alga Hypnea valentiae. The complete genome of the bacterium was sequenced and all detectable natural product gene clusters were analysed. The B. amyloliquefaciens MTCC 12716 genome features polyketide synthase (pks) systems of every known formally classified family, nonribosomal peptide synthetases and hybrid clusters. Comprehensive spectroscopic studies revealed the compounds to possess isobenzofuranyl benzoate and 1H-furopyrano[2,3-c]oxacyclononadecine-6-carboxylate moieties. The identified compounds displayed broad-spectrum bactericidal activity against methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecalis, and drug-resistant strains of Pseudomonas aeruginosa and Klebsiella pneumoniae with minimum inhibitory concentrations (MICs) of <= 1.0 mu g/mL, whereas the standard antibiotics ampicillin and chloramphenicol were active only at concentrations of >= 6.25 mu g/mL. The plausible mechanism of elansolid-type macrolide biosynthesis by trans-AT polyketide synthases through the pks starter unit para-hydroxybenzoic acid was hypothesised, and the structures were correlated with the gene organisation, with the predicted gene cluster comprising 16 genes (similar to 81 kb in size). The best binding poses for each compound with the peptide deformylase (PDF) protein of S. aureus revealed docking scores (>11.30 kcal/mol) greater than actinonin (6.96 kcal/mol), a natural PDF inhibitor. The higher electronic values along with optimum lipophilic parameters support the potential anti-infective properties of the studied macrolides. These antibacterial elansolid-type of polyketide spanned macrolides in marine symbiotic B. amyloliquefaciens could be potential leads for biotechnological and pharmaceutical applications against emerging multidrug-resistant pathogens. (C) 2020 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
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