期刊
INSECTES SOCIAUX
卷 67, 期 2, 页码 317-326出版社
SPRINGER BASEL AG
DOI: 10.1007/s00040-020-00755-8
关键词
Microinjection; Mutagenesis; CRISPR; Cas9; Oenocyte; Fire ant
类别
CRISPR/Cas9 mediated mutagenesis has revolutionized the testing of gene function in both model and non-model organisms. The red imported fire ant, Solenopsis invicta, is the best-studied ant species because of their painful sting, aggressive nature, and their detrimental effects on invaded ecosystems. We have developed a microinjection protocol for CRISPR/Cas9 mutagenesis of fire ant embryos. As a proof-of-principle we generated individuals mosaic for the knockout of Sinv-spitz or Gp-9. We verified that many injected individuals carry mutations, often to high frequency of the cells within the individual. Observations at the first instar larval stage indicate that mutating Sinv-spitz does not produce a loss of oenocyte phenotype, as would have been expected based on the homologous function of the fruit fly, Drosophila melanogaster, spitz. Our success indicates that CRISPR/Cas9 mutagenesis should be a useful technique for studying gene function in the fire ant at the individual and possibly social levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据