期刊
BIORESOURCE TECHNOLOGY
卷 189, 期 -, 页码 36-43出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.02.089
关键词
Lignocellulosic hydrolysate; Ethanol fermentation; Biotin addition; Anti-oxidative activity; Cell viability
资金
- National Natural Science Foundation of China [21176153]
- National Basic Research Program of China (973 program) [2013CB733902]
- Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning [ZXDF080005]
- Program of Shanghai Subject Chief Scientist [14XD1402600]
Commercialization of lignocellulosic ethanol fermentation requires its high titer, but the reactive oxygen species (ROS) accumulation during the bioprocess damaged the cells and compromised this goal. To improve the cellular anti-oxidative activity during non-detoxified corncob residue hydrolysate fermentation, seed cells were prepared to possess a higher level of intracellular biotin pool (IBP), which facilitated the biosyntheses of catalase and porphyrin. As a result, the catalase activity increased by 1.3-folds compared to control while the ROS level reduced by 50%. Cell viability in high-IBP cells was 1.7-folds of control and the final ethanol titer increased from 31.2 to 41.8 g L-1 in batch fermentation. The high-IBP cells were further used for repeated-batch fermentation in the non-detoxified lignocellulosic hydrolysate, and the highest titer and average productivity of ethanol reached 63.7 g L-1 and 1.2 g L-1 h(-1). The results were favorable to future industrial application of this lignocellulosic bioethanol process. (C) 2015 Elsevier Ltd. All rights reserved.
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