期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 145, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2019.112073
关键词
Panax notoginseng; Pathogenic fungi; Mentha haplocalyx; Essential oils; Action mechanisms; Antifungal activity
资金
- National Key Research and Development Program of China Research and Development of Comprehensive Technologies on Chemical Fertilizer and Pesticide Reduction and Synergism [2017YFD0201402]
- National Natural Science Foundation of China [81660626]
- Yunnan Provincial Science and Technology Department-Applied Basic Research Joint Special Funds of Yunnan University of Chinese Medicine [2019FF002(-003), 2017FF116 (-014)]
- Yunnan Applied basic Research Program-Youth Project [2018FB139]
- China Scholarship Council (CSC)
- Yunnan Provincial Department of Education Major Project [2019Y0310]
In order to control the occurrence of Panax notoginseng (Burk.) F.H. Chen diseases, the antifungal effects and action mechanism of essential oils (EOs) against the pathogenic fungi were investigated. The antifungal effect of Mentha haplocalyx Briq. and Schizonepeta tenuifolia Briq. essential oils (EOs) were determined in vivo and in vitro to develop green fungicides to eradicate fungi pathogens affecting Panax notoginseng. The inhibition rates of M. haplocalyx and S. tenuifolia EOs (50 mg/mL) on Fusarium oxysporum, Fusarium solani, Cylindrocarpon destructans, Pythium aphaniciermatum, Botrytis cinerea, Colletorrichurn gloeosporioides and Rhizoctonia solani reached 100.00 % and were assessed by the Oxford cup method. The chemical constituents of M. haplocalyx and S. tenuifolia EOs were analyzed by GC-MS. Low MIC values of M. haplocalyx EO, S. tenuifolia EO and its main components against six fungal strains from P. notoginseng were found, which indicated all have substantial antifungal activity. Furthermore, synergistic effects between the EOs and the agrochemical hymexazol against P. notoginseng pathogens were observed. F. oxysporum was chosen as dominant fungi pathogen to investigate the mechanism of action of M. haplocalyx EO. The antifungal effect of M. haplocalyx EO on F. oxysporum in vitro was investigated. The high M. haplocalyx EO concentrations inhibited the spore germination rates, mycelia dry weights and spore yields and disturbed the mycelia soluble protein and reducing sugar contents; it also increased the cell membrane permeability. M. haplocalyx EO also induced decreased SDH and NADH oxidase activities and caused extreme alterations in ultrastructures compared with the control. Altogether, the antifungal mechanism of M. haplocalyx EO may seemed to consist in penetrating and dissolving the mitochondrial membranes. Overall, M. haplocalyx EO and its major compounds show potential to be used as natural alternatives to commercial fungicides for controlling F. oxysportun infection of P. notoginseng.
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