期刊
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
卷 59, 期 1, 页码 42-51出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.iecr.9b04391
关键词
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资金
- National Natural Science Foundation of China [21576068, 21276060, 21276062, 21306039]
- Natural Science Foundation of Tianjin [16JCYBJC19800]
- Natural Science Foundation of Hebei Province [B2015202082, B2016202027, B2017202056]
- Program for Top 100 Innovative Talents in Colleges and Universities of Hebei Province [SLRC2017029]
- Hebei High Level Personnel of Support Program [A2016002027]
- Graduate Innovation Support Program in Hebei Province [CXZZSS2017024]
Based on glucose oxidase (GOx) and horse-radish peroxidase (HRP), compartmentalization of a bienzyme system was successfully carried out by immobilizing them in hollow metal-organic frameworks via a protein-induced soft-templating pathway. The morphology, structure, and properties of the obtained HRP@H-ZIF-8-GOx (GOx and HRP were immobilized in the shell and cavity of hollow ZIF-8, respectively) were investigated by powder X-ray diffraction, scanning transmission electron microscopy (STEM), and so forth. HRP@H-ZIF-8-GOx showed excellent catalytic activity for the cascade reaction with glucose and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt as the substrates. The temperature adaptability, thermal stability, mechanical stability, and long-term storage stability of HRP@H-ZIF-8-GOx are better than that of HRPGOx@H-ZIF-8 (GOx and HRP were coimmobilized in the cavity of hollow ZIF-8). HRP@H-ZIF-8-GOx was used for the detection of glucose and phenol, which showed excellent detection performance. This proposed platform provides a facile strategy for constructing a compartmental multienzyme system and expands the application potential of multienzyme in biosensor construction, biocatalysis, and environmental field.
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