期刊
BRITISH JOURNAL OF CANCER
卷 114, 期 12, 页码 1367-1375出版社
SPRINGERNATURE
DOI: 10.1038/bjc.2016.121
关键词
pancreatic cancer; circulating tumour cells; biomarker; staging
类别
资金
- UCLA Jonsson Comprehensive Cancer Impact Grant
- NantOmics
- National Institute of Health [R33CA174562, U01 CA198900, R44 CA180482]
Background: Current diagnosis and staging of pancreatic ductal adenocarcinoma (PDAC) has important limitations and better biomarkers are needed to guide initial therapy. We investigated the performance of circulating tumour cells (CTCs) as an adjunctive biomarker at the time of disease presentation. Methods: Venous blood (VB) was collected prospectively from 100 consecutive, pre-treatment patients with PDAC. Utilising the microfluidic NanoVelcro CTC chip, samples were evaluated for the presence and number of CTCs. KRAS mutation analysis was used to compare the CTCs with primary tumour tissue. CTC enumeration data was then evaluated as a diagnostic and staging biomarker in the setting of PDAC. Results: We found 100% concordance for KRAS mutation subtype between primary tumour and CTCs in all five patients tested. Evaluation of CTCs as a diagnostic revealed the presence of CTCs in 54/72 patients with confirmed PDAC (sensitivity = 75.0%, specificity = 96.4%, area under the curve (AUROC) = 0.867, 95% CI = 0.798-0.935, and P<0.001). Furthermore, a cut-off of >= 3 CTCs in 4 ml VB was able to discriminate between local/regional and metastatic disease (AUROC = 0.885; 95% CI = 0.800-0.969; and P<0.001). Conclusion: CTCs appear to function well as a biomarker for diagnosis and staging in PDAC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据