Fully Integrated, Automated, and Smartphone Enabled Point-of-Source Portable Platform With Microfluidic Device for Nitrite Detection

Excess limit of Nitrites, which are prevalent within environmental and physiological systems, have severe detrimental effects, thus point-of-source detection becomes an important requirement to take suitable preventive measures. This paper presents the design and development of a standalone, point-of-source, portable, low-cost, automated and integrated microfluidic system for quick detection and quantification of nitrite. Based on multiphysics simulations, a disposable polydimethylsiloxane (PDMS) microfluidic device was employed to carry out the controlled Griess reaction based assay. A low-cost 3D printed syringe pump was developed to inject the sample and reagent. Photometric detection was employed using light emitting diode (LED) and photodiode. A transimpedance amplifier circuit was designed and fabricated to achieve amplified photodiode output with reduced noise. An off-the-shelf microprocessor was used to integrate the whole system and a smartphone application (app) was developed to control the complete system and store the data. Interaction between the application and the microprocessor was achieved using Bluetooth connectivity. Spectrophotometric validation was performed and a calibration equation was obtained which was used to convert the device voltage output to absorption, through specially programmed android app. All the components were integrated in a 3D printed platform whose virtues such as ease of usage and affordability makes, quantification of nitrite, a simple and real time process wherein the limit of detection and limit of quantification values are found to be 0.07103 ppm and 0.21524 ppm respectively with good repeatability.