期刊
BIORESOURCE TECHNOLOGY
卷 198, 期 -, 页码 395-402出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.09.041
关键词
Composting; Denaturing gradient gel electrophoresis (DGGE); Bacterial diversity; Redundancy analysis (RDA)
资金
- program for the National Natural Science Foundation of China [51378097]
- National Key Technology RD Program [2012BAJ21B02-02]
The aim of this study was to compare the bacterial structure of seven different composts. The primary environmental factors affecting bacterial species were identified, and a strategy to enhance the abundance of uncultured bacteria through controlling relevant environmental parameters was proposed. The results showed that the physical-chemical parameters of each different pile changed in its own manner during composting, which affected the structure and succession of bacteria in different ways. DGGE profiles showed that there were 10 prominent species during composting. Among them, four species existed in all compost types, two species existed in several piles and four species were detected in a single material. Redundancy analysis results showed that bacterial species compositions were significantly influenced by C/N and moisture (p < 0.05). The optimal range of C/N was 14-27. Based on these results, the primary environmental factors affecting a certain species were further identified as a potential control of bacterial diversity. (C) 2015 Elsevier Ltd. All rights reserved.
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