期刊
FUTURE MICROBIOLOGY
卷 14, 期 18, 页码 1545-1557出版社
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2019-0178
关键词
antifungal activity; Candida albicans; chelerythrine; Galleria mellonella model; potential mechanism
类别
资金
- Administration of Traditional Chinese Medicine of Shandong Province, China [2017-166]
- Department of Science and Technology of Shandong Province, China [2017G006038]
Aim: To evaluate whether chelerythrine (CHT) exhibited antifungal activity against Candida albicans in vitro and in vivo and to explore the underlying mechanisms. Materials & methods: Broth microdilution assay and Galleria mellonella model were used to evaluate the antifungal effect in vitro and in vivo, respectively. Mechanism studies were investigated by morphogenesis observation, Fluo-3/AM, DCFH-DA and rhodamine6G assay, respectively. Results: CHT exhibited antifungal activity against C. albicans and preformed biofilms with minimum inhibitory concentrations ranged from 2 to 16 mu g/ml. Besides, CHT protected G. mellonella larvae infected by C. albicans. Mechanisms studies revealed that CHT inhibited hyphal growth, increased intracellular calcium concentration, induced accumulation of reactive oxygen species and inhibited drug transporter activity. Conclusion: CHT exhibited antifungal activity against C. albicans.
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