期刊
BRIEFINGS IN BIOINFORMATICS
卷 18, 期 5, 页码 735-743出版社
OXFORD UNIV PRESS
DOI: 10.1093/bib/bbw057
关键词
single-cell; comparison; differential expression; RNA-seq; reproducibility
资金
- JDRF [2-2013-32]
- Sigrid Juselius foundation
- EU's Horizon research and innovation programme [675395]
- University of Turku Graduate School UTUGS
We compared five statistical methods to detect differentially expressed genes between two distinct single-cell populations. Currently, it remains unclear whether differential expression methods developed originally for conventional bulk RNA-seq data can also be applied to single-cell RNA-seq data analysis. Our results in three diverse comparison settings showed marked differences between the different methods in terms of the number of detections as well as their sensitivity and specificity. They, however, did not reveal systematic benefits of the currently available single-cell-specific methods. Instead, our previously introduced reproducibility-optimization method showed good performance in all comparison settings without any single-cell-specific modifications.
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