期刊
FREE RADICAL RESEARCH
卷 54, 期 2-3, 页码 126-136出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715762.2020.1715965
关键词
27-Hydroxycholesterol; breast cancer; matrix metalloproteinase 9; reactive oxygen species; RECK
资金
- National Natural Science Foundation of China [81573183, 81673205]
- Major Programme of Natural Science Research of Jiangsu Higher Education Institutions [15KJA330001]
- Priority Academic Programme Development of Jiangsu Higher Education Institutions (PAPD)
- Centre for Global Health, School of Public Health, Nanjing Medical University
Breast cancer is an important and common tumour among women worldwide. We previously showed that 27-hydroxycholesterol (27HC) promoted the invasion and migration of breast cancer cells and activated signal transducer and activator of transcription 3 (STAT-3) signalling through reactive oxygen species (ROS). However, the regulation of STAT-3 signalling by ROS needs to be further explored. Here, we showed that 27HC caused the accumulation of cellular ROS, which upregulated matrix metalloproteinase 9 (MMP9) and increased the invasive ability of MCF7 and T47D cells. 27HC decreased the protein and mRNA levels of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) in a time- and dose-dependent manner in MCF7 and T47D cells. RECK downregulation was mediated by 27HC-induced DNA methylation via ROS in MCF7 cells. RECK knockdown increased the activity and mRNA levels of MMP9, and promoted the invasion of MCF7 cells. We also found RECK knockdown upregulated the level of p-STAT-3 in MCF7 cells. Furthermore, overexpression of RECK attenuated 27HC-induced invasion in MCF7 cells. RECK overexpression also inhibited p-STAT-3 upregulation induced by 27HC. Collectively, the results showed that DNA methylation induced by 27HC via ROS downregulated RECK, thereby activating the STAT-3 signalling pathway. RECK could serve as a novel target mediating the effect of 27HC on breast cancer.
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