期刊
FOOD RESEARCH INTERNATIONAL
卷 128, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.foodres.2019.108763
关键词
Principal component analysis; Response surface methodology; Anthocyanins; Unconventional foods; Antihypertensive activity; Inhibitor of DNA strand scission
资金
- PROAP/CAPES
- Fundacao Araucaria
- Conselho Nacional de Desenvolvimento Cientffico e Tecnologico (CNPQ) [302763/2014-7, 305804/2017-0, 303188/2016-2]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) [001]
The purpose of this study was to use a statistical approach to optimise the experimental conditions regarding the extraction of bioactive compounds, and to analyse the in vitro functional properties of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of Clitoria ternatea petals. The results showed that the factors of temperature and time influenced the extraction of phenolic compounds, antioxidant activity and the physicochemical parameters. Simultaneous optimisation showed that the same levels of bioactive compounds were extracted when using temperatures from 11.7 to 68.3 degrees C and times from 8.47 to 51.12 min. Principal component analysis revealed the experimental conditions that provided the extraction producing the highest level of phenolic content (40 degrees C/30 min). The CLE showed antimicrobial activity; protective effect against hemolysis of erythrocytes; inhibition of alpha-amylase, alpha-glucosidase and angiotensin-I-converting (ACE-D enzymes; and inhibition of lipid peroxidation. The CLE and PPE demonstrated oxygen radical absorption capacity; inhibition of DNA strand scission; inhibition of LDL cholesterol oxidation; intracellular antioxidant activity against reactive oxygen species ( > 100 mu g/mL); and no cytotoxicity (IC50, GI(50) and LC50 > 900 mu g/mL) against A549, HCT8 and IMR90 cell lines.
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