Polyamide modified with green tea extract for fresh minced meat active packaging applications

New antioxidant polyamide was prepared by total immersion in active extract for 48 h. Its antioxidant performance was tested using DPPH (IC50 = 270 +/- 21 mu g/g) and ORAC (1.52 mu mol Trolox/g). In vivo study was done using fresh minced meat stored at 4 degrees C and analysed after 0, 6, 13, 19, 23 days. After 23 days metmyoglobin (MetMb(BK) = 31.3 +/- 2.9% and MetMb(AOX) = 25.9 +/- 0.8%), CIE L*a*b* (a*(BK) = 15.0 +/- 0.4 and a*(AOX) = 16.6 +/- 0.3) and TBARS (MDA(BK) = 0.0060 +/- 0.0003 mu g/g and MDA(AOX) = 0.0044 +/- 0.0002 mu g/g) showed an improvement of meat shelf life. The results showed that this active film protected meat during 23 days. Migration study to food simulants was done by UPLC (R)-QqQ-MS and UPLC (R)-ESI-Q-TOF-MSE. A decrease of oligomers migration (for caprolactam n = 3: BK = 0.050 +/- 0.004 mg/Kg; AOX = 0.019 +/- 0.001 mg/Kg) was found. Industrial relevance: Nowadays, food industry is focused on improving shelf life of products by controlling its lipid oxidation using natural antioxidants. The obtained results la us design a new active packaging based on natural antioxidants for extending the shelf life of fresh minced meat at industrial scale.