期刊
FOOD CHEMISTRY
卷 307, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125645
关键词
Ca2+; Gold nanoparticles; Graphene; Plasma oxidation; Troponin C
资金
- National Natural Science Foundation of China [31871827]
- Fundamental Research Funds for the Central Universities [Y0201900390, KYDZ201902, CARS-35]
- Jiangsu Agriculture Science and Technology Innovation Fund [CX(19)2018]
A simple electrochemical sensor was developed to determine the concentration of Ca2+ in meat. Graphene was treated with oxygen plasma for 10 s and 30 s comparing with the pristine graphene. Through analyzing morphology and chemical composition, the graphene with the lowest defect density was chosen to mix with bovine serum albumin molecule-functionalized gold nanoparticles. It was interesting that only a few gold nanoparticles were trapped in the graphene with 10 s plasma treatment. Then, under the optimal condition measured, the limit of detection was detected as 3.9 x 10(-8) M with a linear relationship from 5 x 10(-8) to 3 x 10(-4) M. Finally, the proposed electrochemical method was applied to detect Ca2+ in the pork sample with stability and reproducibility verified by parallel detections. Thus, the proposed method demonstrates its potential for effectively detecting Ca2+ in meat and prominently reduces time consumption on operations and pretreatments.
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