4.7 Article

Effects of collagenase type I on the structural features of collagen fibres from sea cucumber (Stichopus japonicus) body wall

期刊

FOOD CHEMISTRY
卷 301, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125302

关键词

Sea cucumber (Stichopus japonicus); Autolysis; Collagen fibres; Collagenase; Degradation

资金

  1. National Natural Science Foundation of China [U1808203, 30901124]
  2. National Key R&D Program of China [2018YFD0901002]
  3. Natural Science Foundation of Liaoning Province of China [20180540062]
  4. Project of Distinguished Professor of Liaoning Province [2015-153]

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The autolysis of sea cucumber is caused by depolymerisation of collagen fibres and unfolding of fibrils. In order to highlight the role of collagenase in sea cucumber autolysis, collagen fibres from sea cucumber were hydrolysed with collagenase type I. Electron microscopy (EM) results indicated the collagenase caused partial depolymerisation of collagen fibres into fibrils due to the fracture of proteoglycan interfibrillar bridges, as well as uncoiling of collagen fibrils. Chemical analysis and SDS-PAGE both indicated collagenase induced a time-dependent release of glycosaminoglycans (GAGs) and soluble proteins, which further demonstrated the degradation of proteoglycan interfibrillar bridges. Collagenase also degraded collagens by releasing soluble hydroxyproline (Hpy), with the dissolution rate of Hyp reaching 11.11% after 72 h. Fourier transform infrared analysis showed that collagenase caused the reduction of intermolecular interactions and structural order of collagen. Hence, collagenase participated in the autolysis of sea cucumber by deteriorating both macromolecular and monomeric collagens.

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