期刊
FOOD CHEMISTRY
卷 300, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125177
关键词
Point-of-care (POC) testing; Mercury; 3D-printing; DNAzyme; Colorimetric detection; Drinking Water
资金
- Main Research Program of the Korea Food Research Institute (KFRI) - Ministry of Science and ICT of South Korea [E0192102-01]
A point-of-care testing chip was developed for the colorimetric detection of inorganic mercury ion (Hg2+). The disposable chip fabricated by three-dimensional printing technology contains DNAzymes produced by rolling circle amplification (RCA); a color change caused by the enzymatic reaction between DNAzymes and the peroxidase substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is measured using a portable spectrophotometer. In the turn-off-type RCA reaction, the annealing of the T(12) primer that initiates the RCA reaction is blocked by the interaction of thymine with Hg2+; thus, the amount of amplified DNAzymes causing a color change is decreased depending on Hg2+ concentration. The colorimetric signal is enhanced by amplifying double-repeat DNAzymes from a circular DNA template. The chip detected Hg2+ in tap drinking water samples with high sensitivity (lowest validated value: 3.6 mu g/L) and showed better selectivity, precision, and reproducibility than conventional analysis instruments. This low-cost easy-to-use platform can reduce the risk of accidental Hg2+ poisoning.
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