期刊
FISH & SHELLFISH IMMUNOLOGY
卷 98, 期 -, 页码 595-604出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2020.01.056
关键词
Grouper; Nervous necrosis virus; Deep sequencing; RNAseq; Proteome; LC-MS
资金
- 2018 Fujian Provincial Oceanic economic development projects [FJHJF-L-2018-3]
- Special Project for Basic Scientific Research of Public Welfare Institutes in Fujian Province [2019R1013-10, 2019R1013-2]
- Special funds for structural adjustment of the oceans and fisheries in Fujian Province [2019HYJG02]
Grouper (Epinephelus coioides) is an important commercial maricultural fish, which suffers from nervous necrosis virus (NNV) infection. The molecular mechanisms underlying the pathogenesis of the viral infection are not clear. In this study, we combined deep RNA sequencing and label-free mass spectrum for the first time to analyze the transcriptomic and proteomic profiles in infected/dead, infected/survival (persistent), and infection-free (control)orange-spotted groupers in the larval stage. Further analyses showed that the transcriptome and proteome changed dramatically among the three distinct groups, especially differentially-expressed genes in the infected/dead and infected/survival larvae enriched for pathways related to immune response. Notably, the overlapped genes between transcriptomes and proteomes identified that genes related to collagen synthesis and adhesion molecules were enhanced in the persistent (infected/survival) stage, which might contribute to suppressing the acute and lethal immune responses upon NNV infection. These transcriptomic and proteomic datasets enable the investigation of molecular mechanisms underlying NNV infection, thus may help further development of molecular breeding in marine fishery.
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