期刊
FISH & SHELLFISH IMMUNOLOGY
卷 97, 期 -, 页码 125-134出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.11.069
关键词
Epinephelus coioides; Hsp22; SGIV; Vibrio alginolyticus; Apoptosis
资金
- National Natural Science Foundation of China [31772877, 31330082, 41876162, 41706186]
- Marine Fisheries Bureau Key Funds and Marine Projects [GDME-2018C002]
- National Key R&D Program of China [2018YFD0900501, 2018YFC0311302]
- China Agricultural Research System [CARS-47G16]
- China Modern Agricultural Industry Technology System [CARS-47-18]
- foundation of Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences [2018011005]
Heat shock protein 22 (Hsp22) is an important regulatory factor response to various stresses in mammals. In this study, the full length cDNA of Epinephelus coioides Hsp22, which was 1680bp in length, with a 289 bp 5' UTR, a 725 bp 3'UTR, and a 666 bp open reading frame encoding 221 amino acids, was obtained. E. coioides Hsp22 contains a highly conserved alpha-crystallin domain. E. coioides Hsp22 mRNA was detected in all tissues examined by quantitative real-time PCR, with the highest expression in blood, followed by the spleen, skin, gill, head kidney, muscle, heart, liver, trunk kidney, stomach, pyloric caeca, intestine, brain and thymus. The expression patterns of E. coioides Hsp22 response to infection with Singapore grouper iridovirus (SGIV) and Vribro alginolyticus, the important pathogens of E. coioides, were studied. The expression levels of the gene were up-regulated in the tissues examined. Subcellular localization analysis demonstrated that E. coioides Hsp22 was distributed in both the cytoplasm and nucleus. In addition, E. coioides Hsp22 significantly inhibited the SGIV-induced cell apoptosis. In summary, the E. coioides Hsp22 might play a critical role in pathogenic stimulation.
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