期刊
FASEB JOURNAL
卷 34, 期 3, 页码 3631-3645出版社
WILEY
DOI: 10.1096/fj.201901808RRR
关键词
apoptosis; FSH; HIF-1 alpha; hypoxia; mitophagy; Parkin; PINK1; porcine granulosa cells
资金
- National Natural Science Foundation of China (NSFC) [31630072, 31601939, 31972564]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [DKQB201903]
- fundamental research funds for the central universities [KJQN201705]
In developing follicles, the granulosa cells (GCs) live in a hypoxic environment due to the devoid of blood supply. Upon hypoxic conditions, several types of mammalian cells have been reported to undergo apoptosis. Follicle-stimulating hormone (FSH) is known as the primary survival factor for antral follicles by preventing GCs apoptosis. Mitophagy is a type of organelle-specific autophagy that removes damaged or stressed mitochondria to maintain cellular health. This study provides the first evidence suggesting that FSH-mediated mitophagy protected porcine GCs from hypoxia-induced apoptosis. Our data showed that the GCs apoptosis caused by mitochondrial pathway upon hypoxia stress was markedly attenuated after FSH treatment, which was correlated with enhanced activation of mitophagy. Interestingly, FSH also stimulated mitochondrial biogenesis as suggested by increased expression of mitochondrial transcription factor A and nuclear respiratory factor 1 during hypoxia exposure. Notably, the protein level of hypoxia inducible factor-1 alpha (HIF-1 alpha) was significantly increased in hypoxic GCs following FSH treatment, accompanied by elevated mitophagic activity and dampened apoptotic signaling. Blocking HIF-1 alpha inhibited mitophagy and restored hypoxia-induced apoptosis despite FSH treatment. Importantly, FSH promoted the expression of serine/threonine kinase PTEN induced putative kinase 1 (PINK1) and the E3 ligase Parkin during hypoxia stress through a HIF-1 alpha dependent manner. This induced the mitophagic clearance of damaged mitochondria, hence inhibiting apoptosis by reducing cytochrome c releasing. The inhibition of HIF-1 alpha and/or PINK1 using inhibitor or RNAi further confirmed the role of the FSH-HIF-1 alpha-PINK1-Parkin-mitophagy axis in suppressing GC apoptosis under hypoxic conditions. These findings highlight a novel function of FSH in preserving GCs viability against hypoxic damage by activating HIF-1 alpha-PINK1-Parkin-mediated mitophagy.
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