期刊
FASEB JOURNAL
卷 33, 期 12, 页码 14668-14679出版社
WILEY
DOI: 10.1096/fj.201901500R
关键词
retina; inflammation; vessels
资金
- Deutsche Forschungsgemeinschaft (DFG) [FE 969/2-1, HA 1755/10-1, IRTG 1874 DIAMICOM-SP2]
- Deutsche Diabetes Gesellschaft (DDG)
- European Foundation for the Study of Diabetes (EFSD)
- Novartis
The aim of this study is to investigate the vascular outcome after intravitreal mesenchymal stem cell (MSC) administration in rats without or with damage to the neurovascular unit [transgenic (TGR) rats]. Male Sprague-Dawley (SD) and TGR rats received an intravitreal injection of 2 x 10(4) rat bone marrow-derived MSCs (BMSCs) or human adipose-derived stem cells (ASCs) at postnatal d 30. After 4 wk, vasculature, neuronal function, and gene expression in the retinas were evaluated using retinal morphometry, electroretinography, immunofluorescence, Western blot, and quantitative PCR. Intravitreal administration of rat BMSCs and human ASCs in both SD and TGR eyes induced cataract, loss of pericytes, and increased formation of acellular capillaries. BMSCs remained in the vitreous cavity and did not migrate into the retinas. Intravitreal administration of BMSCs impacted retinal neuronal function in neither SD nor TGR rats. Retinal glial activation, elevation of IL-1 beta, C3, arginase 1, and heat shock protein 90 were detected in BMSC-injected SD rats. Intravitreal administration of MSCs induces cataract, retinal vasoregression, activation of retinal glial cells, and inflammatory response in rat eyes.
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