期刊
EXPERIMENTAL CELL RESEARCH
卷 389, 期 1, 页码 -出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2020.111856
关键词
Colorectal cancer; Carboxylesterase-2; Oxaliplatin; Chemotherapy resistance; PI3K signaling pathway
资金
- National Natural Science Foundation of China [81472777]
- Science Foundation of the Fujian Province, China [2017J01296]
- National Clinical Key Specialty Construction Project (General Surgery) of China [2012-649]
- Joint Funds for the innovation of science and Technology, Fujian province [2018Y9021]
CES-2 (carboxylesterase-2) belongs to the carboxylesterase gene family, which plays crucial roles in lipid mobilization and chemosensitivity to irinotecan. However, its role in chemosensitivity to oxaliplatin (L-OHP) remains unclear. Herein, L-OHP-resistant cells (HCT-116L and RKOL) were established by increasing the concentration of L-OHP. The results showed that CES2 expression was upregulated in L-OHP-resistant tissues and cells lines (both P < 0.01). Low expression of CES2 correlated with a better survival, and the results were further confirmed in the R2 platform: a biologist friendly web-based genomics analysis and visualization application. Downregulation of CES2 suppressed cell proliferation, induced apoptosis and reversed L-OHP resistance by medicating the PI3K signaling pathway in L-OHP-resistant cells. However, both PI3K inhibitor (LY294002) and activator (IGF-1) could not medicate CES2 expression. These findings indicated that CES2 may be utilized as a novel biomarker and therapeutic target for L-OHP resistance in CRC treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据