期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 868, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ejphar.2019.172854
关键词
Rheumatoid arthritis (RA); Sigesbeckiae herba (SH); Leocarpinolide B; Nuclear factor erythroid 2-related factor 2 (Nrf2); Nuclear transcription factor-kappa B (NR-kappa B)
资金
- National Natural Science Foundation of China [NSFC] [81470170]
- Science and Technology Development Fund, Macau SAR [0096/2019/A2]
- Research Committee of the University of Macau [MYRG2017-00178-ICMS, MYRG2018-00043-ICMS]
Macrophages-mediated inflammation is involved in the regulation of rheumatoid arthritis (RA). Sigesbeckiae Herba (SH) has been traditionally used for rheumatism. However, the bioactive ingredients of SH are still unclear. Recently, we isolated a compound (Leocarpinolide B, LB) from SH and identified its potent anti-inflammatory and antioxidant effects on RAW264.7 macrophages for the first time. LB effectively inhibited excessive production of nitric oxide (NO), prostaglandin E2 (PGE(2)), cytokines (IL-6, TNF-alpha and MCP-1), and the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) in lipopolysaccharide (LPS)-induced RAW264.7 cells. LB blocked the degradation of inhibitor of kappa B (I kappa B alpha) and translocation of nuclear factor kappa B (NF-kappa B) p65. Additionally, LB reduced the intracellular reactive oxygen species, and increased the expression of heme oxygenase-1 (HO-1) and the translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) in the presence or absence of LPS. The results suggested that LB might be one of the bioactive components of SH, and be potential for the treatment of RA and valuable to be further investigated.
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