期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 133, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2019.109459
关键词
2-Phenylethanol; Metabolic engineering; Pichia pastoris
资金
- National Science Foundation of Jiangxi Province [2018ACB21045, 20192BCBL23012]
2-Phenylethanol (2-PE) is an important flavour and fragrance compound with a rose-like odor, which is widely used in cosmetics and food industries. Natural 2-PE is costly and cannot meet the market demand due to the relative low content of 2-PE in the plants. Thus, there is an increasing interest in the search for alternative routes for 2-PE production. Here we demonstrate the engineering of Pichia pastoris to produce 2-PE directly from simple sugars for the first time. We first demonstrated that improving downstream pathway from phenylpyruvate to 2-PE by overexpressing ARO10 and ADH6 could increase the biosynthesis of 2-PE. Then several genetic engineering strategies were developed to increase phenylpyruvate availability to improve 2-PE production. 1169 mg/L of 2-PE was accumulated in the final engineered strain. This study showed the potential of P. pastoris as a host strain to produce industrially interested 2-PE by metabolic engineering.
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