Simultaneous detection of mercury (II), lead (II) and silver (I) based on fluorescently labelled aptamer probes and graphene oxide

We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg2+, Pb2+ and Ag+ based on fluorescently labelled nucleic acid aptamer probes and graphene oxide (GO). By this method, three nucleic acid aptamer probes (P-Hg, P-Pb, P-Ag) were designed. The carboxyl fluorescein (FAM), tetramethyl-6-carboxyrhodamine (TAMRA) and cyanine-5 (Cy-5) were respectively selected as fluorophore of aptamer probes, and GO was chosen as quencher. In general, these probes were on free single-stranded state and adsorbed on the surface of GO via pi-pi interactions, which brought fluorophores of probes and GO into close proximity. Due to the fluorescence resonance energy transfer occurred between fluorophores and GO, the fluorescence was quenched and fluorescence signals were all weak. Under the optimal condition, fluorescence intensities of three fluorophores exhibited a good linear dependence on corresponding ions concentration. The detection limit for Hg2+, Pb2+ and Ag+ were 0.2, 0.5 and 2 nmol/L (3 sigma, n = 11). Average recoveries of this method were 97.56-104.92%, which indicated the method had a high accuracy and low detection limit. In addition, this proposed method has good selectivity, and there was no crosstalk effect among these probes.

Automated summary

The developed method uses fluorescently labelled nucleic acid aptamer probes and graphene oxide for simultaneous detection of Hg2+, Pb2+ and Ag+. It shows high accuracy, low detection limits, good selectivity, and no crosstalk among the probes.