Investigations on the phytotoxicity of perfluorooctanoic acid in Arabidopsis thaliana

Environmental contamination by perfluorooctanoic acid (PFOA) has raised concerns for years. Yet, little information on its phytotoxic effects and underlying mechanisms in higher plants is available. To this end, comparative analyses of the responses to PFOA exposure between shoots and roots in the model plant species Arabidopsis thaliana were performed at the physiological and molecular levels. Our results showed that PFOA exposure reduced Arabidopsis biomass in a dose-related manner, and shoot growth was more sensitive to PFOA than root growth. Consistently, PFOA accumulation and the levels of several metal elements, including Zn, Ca, Cu, and K, in addition to Fe, were more substantially affected in the shoots than in the roots. Transcriptomic analysis further showed that the shoot transcriptional profile was distinguishable from that of roots upon PFOA exposure. Nevertheless, some overlapping genes were present between the shoots and roots, mainly including transporter genes, Fe-deficiency-responsive genes, and oxidative stress-related genes. More importantly, a comparative analysis of ROS-associated genes in combination with other oxidative stress assays pointed out that PFOA triggered certain oxidative stress-associated events more strongly in shoots than in roots. Overall, the results demonstrated that PFOA exposure caused alterations in PFOA distribution, metal element balance, reconfiguration of transcriptomes, and induction of oxidative stress in a tissue-dependent manner in Arabidopsis thaliana.