期刊
ENVIRONMENTAL MICROBIOLOGY
卷 22, 期 8, 页码 2996-3004出版社
WILEY
DOI: 10.1111/1462-2920.14975
关键词
-
类别
资金
- Commissariat a l'Energie Atomique et aux Energies Alternatives (France)
- Agence Nationale de la Recherche program 'Phylopeptidomics' [ANR-17-CE18-0023-01]
Differential shotgun proteomics identifies proteins that discriminate between sets of samples based on differences in abundance. This methodology can be easily applied to study (i) specific microorganisms subjected to a variety of growth or stress conditions or (ii) different microorganisms sampled in the same condition. In microbiology, this comparison is particularly successful because differing microorganism phenotypes are explained by clearly altered abundances of key protein players. The extensive description and quantification of proteins from any given microorganism can be routinely obtained for several conditions within a few days by tandem mass spectrometry. Such protein-centred microbial molecular phenotyping is rich in information. However, well-designed experimental strategies, carefully parameterized analytical pipelines, and sound statistical approaches must be applied if the shotgun proteomic data are to be correctly interpreted. This minireview describes these key items for a quick molecular phenotyping based on label-free quantification shotgun proteomics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据