期刊
ENVIRONMENTAL MICROBIOLOGY
卷 22, 期 2, 页码 726-737出版社
WILEY
DOI: 10.1111/1462-2920.14865
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资金
- Strategy and Innovation Grant from the Free State of Thuringia [41-5507-2016]
- Leibniz Research Cluster InfectoOptics [SAS-2015-HKI-LWC]
- Collaborative Research Centre AquaDiva of the Friedrich Schiller University Jena - Deutsche Forschungsgemeinschaft [CRC 1076 AquaDiva]
- Thuringer Ministerium fur Wirtschaft, Wissenschaft und Digitale Gesellschaft (TMWWDG) [2016 FGI 0024]
- Ministerium fur Kultur und Wissenschaft des Landes Nordrhein-Westfalen
- LABGeM (CEA/Genoscope CNRS UMR8030)
- France Genomique
- French Bioinformatics Institute national infrastructures (Investissement d'Avenir program) [ANR-10-INBS-09, ANR-11-INBS-0013]
Subsurface ecosystems like groundwater harbour diverse microbial communities, including small-sized, putatively symbiotic organisms of the Candidate Phyla Radiation, yet little is known about their ecological preferences and potential microbial partners. Here, we investigated a member of the superphylum Microgenomates (Cand. Roizmanbacterium ADI133) from oligotrophic groundwater using mini-metagenomics and monitored its spatio-temporal distribution using 16S rRNA gene analyses. A Roizmanbacteria-specific quantitative PCR assay allowed us to track its abundance over the course of 1 year within eight groundwater wells along a 5.4 km hillslope transect, where Roizmanbacteria reached maximum relative abundances of 2.3%. In-depth genomic analyses suggested that Cand. Roizmanbacterium ADI133 is a lactic acid fermenter, potentially able to utilize a range of complex carbon substrates, including cellulose. We hypothesize that it attaches to host cells using a trimeric autotransporter adhesin and inhibits their cell wall biosynthesis using a toxin-antitoxin system. Network analyses based on correlating Cand. Roizmanbacterium ADI133 abundances with amplicon sequencing-derived microbial community profiles suggested one potential host organism, classified as a member of the class Thermodesulfovibrionia (Nitrospirae). By providing lactate as an electron donor Cand. Roizmanbacterium ADI133 potentially mediates the transfer of carbon to other microorganisms and thereby is an important connector in the microbial community.
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