期刊
BRAIN STRUCTURE & FUNCTION
卷 222, 期 5, 页码 2359-2378出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00429-016-1346-2
关键词
Globus pallidus; Basal ganglia; Dopamine neuron; Substantia nigra
资金
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT) [25282247, 15K12770, 26350983, 16H01622, 25560435, 16H02840, 16H01623, 16K13115, 16H06543, 26112001, 152107008]
- Grants-in-Aid for Scientific Research [15K06732, 16H01611, 16H03299, 16H01622, 16K13115, 26112006, 16K21735, 16H01623, 15H01434, 16H06276, 16H06543, 25560435, 16H06535, 25282247, 16H02454, 16H02840, 26112001, 15K21715, 26112002, 26350983, 15K12770] Funding Source: KAKEN
In the present study, we generated a novel parvalbumin (PV)-Cre rat model and conducted detailed morphological and electrophysiological investigations of axons from PV neurons in globus pallidus (GP). The GP is considered as a relay nucleus in the indirect pathway of the basal ganglia (BG). Previous studies have used molecular profiling and projection patterns to demonstrate cellular heterogeneity in the GP; for example, PV-expressing neurons are known to comprise approximately 50% of GP neurons and represent majority of prototypic neurons that project to the subthalamic nucleus and/or output nuclei of BG, entopeduncular nucleus and substantia nigra (SN). The present study aimed to identify the characteristic projection patterns of PV neurons in the GP (PV-GP neurons) and determine whether these neurons target dopaminergic or GABAergic neurons in SN pars compacta (SNc) or reticulata (SNr), respectively. We initially found that (1) 57% of PV neurons co-expressed Lim-homeobox 6, (2) the PVGP terminals were preferentially distributed in the ventral part of dorsal tier of SNc, (3) PV-GP neurons formed basket-like appositions with the somata of tyrosine hydroxylase, PV, calretinin and cholecystokinin immunoreactive neurons in the SN, and (4) in vitro wholecell recording during optogenetic photo-stimulation of PVGP terminals in SNc demonstrated that PV-GP neurons strongly inhibited dopamine neurons via GABA(A) receptors. These results suggest that dopamine neurons receive direct focal inputs from PV-GP prototypic neurons. The identification of high-contrast inhibitory systems on dopamine neurons might represent a key step toward understanding the BG function.
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