期刊
BRAIN RESEARCH
卷 1634, 期 -, 页码 57-67出版社
ELSEVIER
DOI: 10.1016/j.brainres.2015.12.057
关键词
Amitriptyline; Antidepressant; Astrocyte; Microglia; BDNF; Major depressive disorder
资金
- JSPS [23790297, 25870461, 15K09819]
- Naito Foundation, Japan
- SENSHIN Medical Research Foundation
- Promotion Project for Research of Detection System of Biological Reaction and Life-maintenance Mechanism at Hiroshima University
- Grants-in-Aid for Scientific Research [23790297, 25870461, 15K09819] Funding Source: KAKEN
A significant role of brain-derived neurotrophic factor (BDNF) has been previously implicated in the therapeutic effect of antidepressants. To ascertain the contribution of specific cell types in the brain that produce BDNF following antidepressant treatment, the effects of the tricyclic antidepressant amitriptyline on rat primary neuronal, astrocytic and microglial cortical cultures were examined. Amitriptyline increased the expression of BDNF mRNA in astrocytic and microglial cultures but not neuronal cultures. Antidepressants with distinct mechanisms of action, such as clomipramine, duloxetine and fluvoxamine, also increased BDNF mRNA expression in astrocytic and microglial cultures. There are multiple BDNF mRNA variants (exon I, IIA, IV and VI) expressed in astrocytes and microglia and the variant induced by antidepressants has yet to be elaborated. Treatment with antidepressants increased the expression of exon I, IV and VI in astrocyte and microglia. Clomipramine alone significantly upregulated expression of exon IIA. The amitriptyline-induced expression of both total and individual BDNF mRNA variants (exon I, IV and VI) were blocked by MEK inhibitor U0126, indicating MEK/ERK signaling is required in the expression of BDNF. These findings indicate that non-neural cells are a significant target of antidepressants and further support the contention that glial production of BDNF is crucial role in the therapeutic effect of antidepressants. The current data suggest that targeting of glial function could lead to the development of antidepressants with a truly novel mechanism of action. (C) 2016 Elsevier B.V. All rights reserved.
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