期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 190, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2020.110180
关键词
Allelopathic effect; Co-culture; Petroleum ether extract; Anti-Algal substances; Mechanisms
资金
- National Natural Science Foundation of China [41876078]
- Shandong Provincial Natural Science Foundation of China [ZR2018MD016]
- Public Science and Technology Research Funds Projects of Ocean [201505034]
- Special Fund for Basic Scientific Research in Central Colleges and Universities [201713059]
This paper studied the allelopathic effect of Cylindrotheca closterium on the growth of Prorocentrum donghaiense, the model of harmful algal blooms in aquatic environment, by the co-culture tests and bioassay-guided fraction methods. The growth of P. donghaiense in co-cultures was observably suppressed by C. closterium, and P. donghaiense biomass in C/P = 3: 1 group increased slowly with a low growth rate of 0.18 d(-1) after 4 days. Petroleum ether (PE) extract derived from C. closterium filtrates was isolated by C18 column and the allelopathy of all isolated fractions for P. donghaiense was investigated. After 96 h cultivation, the inhibition ratio of PE-III and PE-VIII fractions on P. donghaiense could reach up to 70.2% and 64.3% at the concentration of 10-fold when compared to control, while the other fractions displayed relatively low inhibitory effects on P. donghaiense. PE-III and PE-VIII fractions also decreased the chlorophyll content and maximum quantum yield of photosystem II (Fv/Fm) of P. donghaiense cells. The activities of superoxide dismutase (SOD), one of antioxidant enzymes, reduced around 8.3% and 13.7% following exposure to 2-fold PE-III and PE-VIII, and was significantly decreased following higher exposure concentrations. After 96 h of 10-fold PE-III and PE-VIII treatments, Catalase (CAT) activity reduced to 44.86% and 46.42% of that observed in the control group. At the same time, a significant increase in malondialdehyde (MDA) contents was observed. These findings suggested that PE-III and PE-VIII fractions contained main allelochemicals and possibly acted as promising algistatic agents for emergency handling of P. donghaiense blooms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据