期刊
DYES AND PIGMENTS
卷 172, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2019.107820
关键词
Peroxynitrite; NIR fluorescent probe; Large Stokes shift; Bioimaging; Living cells and animals
资金
- National Natural Science Foundation of China [21672080, 21472066]
- Fundamental Research Funds for the Central Universities [CCNU19TS007]
Detection and imaging of peroxynitrite (ONOO-) in living systems has attracted considerable attention in recent years due to its biological significance. In this paper, a new highly selective and sensitive near-infrared (NIR) fluorescent turn-on probe for ONOO- was developed. This probe uses a dicyanoisophorone-based fluorescent dye as the fluorophore and alpha-ketoamide as the reaction site. In the presence of ONOO-, the probe exhibits notable fluorescence enhancement at 660 nm with rapid response (< 2 s), large Stokes shift (200 nm), and a low detection limit (81 nM). In addition, MTT assays indicated that this probe is of low cytotoxicity in cells, and bioimaging experiments demonstrated that the probe could be applied for imaging of ONOO- in living cells, zebrafish and mice with good imaging performance.
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