期刊
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
卷 46, 期 2, 页码 236-245出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/03639045.2020.1716374
关键词
Polymeric nanoparticles; colon-targeted release; in vitro permeability; caco-2 cells; triple co-culture model; everted gut sac
资金
- Sao Paulo Research Foundation (FAPESP, Brazil) [2014/50928-2]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico'' (CNPq, Brazil) [465687/2014-8]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao de Amparo e Pesquisa do Estado de Sao Paulo (FAPESP)
- Norte Portugal Regional Operational Programme (NORTE 2020) [NORTE-01-0145-FEDER-000012]
- COMPETE 2020 -Operacional Programme for Competitiveness and Internationalisation (POCI), under the PORTUGAL 2020 Partnership Agreement, through the FEDER - Fundo Europeu de Desenvolvimento Regional
- FCT - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia e Ensino Superior [UID/BIM/04293/2019]
- Fundacao para a Ciencia e a Tecnologia (FCT), Portugal [SFRH/BD/118721/2016]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/118721/2016] Funding Source: FCT
Nanoparticles based on gellan gum/pectin blends were designed for colon-targeted release of resveratrol (RES). Their impact on drug release rates and permeability were evaluated using Caco-2 cell model and mucus secreting triple co-culture model. Polymeric nanoparticles (PNP) were successfully prepared by nebulization/ionotropic gelation, achieving high drug loading (>80%). PNP were spherical with a low positive charge density (+5mV) and exhibited diameters of around 330 nm. Developed PNP were able to promote effective modulation of drug release rates, so that only 3% of RES was released in acidic media over 2 h, and, in pH 6.8, the drug was released in a sustained manner, reaching 85% in 30 h. The permeability of RES-loaded PNP in the Caco-2 model was 0.15%, while in the triple co-culture model, in the presence of mucus, it reached 5.5%. The everted gut sac experiment corroborated the low permeability of RES-loaded PNP in the presence or absence of mucus and highlighted their high ability to interact with the intestinal tissue. Results indicate that the novel PNP developed in this work are safe and promising carriers for controlled delivery of RES at the colon.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据