期刊
BRAIN RESEARCH
卷 1649, 期 -, 页码 123-131出版社
ELSEVIER
DOI: 10.1016/j.brainres.2016.08.012
关键词
Senkyunolide I; Microglial cells; Oxygen-glucose deprivation/reoxygenation; TLR4/NF-kappa B pathway; HSF-1/Hsp70
资金
- Chinese Ministry of Education [211060]
- Sci-Tech Innovation Item of Shanghai Education Committee [12ZZ124]
- Research Fund for the Doctoral Program of Higher Education of China [20123107120009]
Over-activated microglia during stroke has been documented to aggravate brain damage. Our previous studies showed that senkyunolide I (SEI) exerted anti-inflammatory effects against endotoxin insult in vitro and ameliorative effects on cerebral ischemia/reperfusion (I/R) injury in vivo. Using oxygen-glucose deprivation/reoxygenation (OGD/R) to mimic stroke, we here investigated the anti-inflammatory effect of SEI on microglial cells and explored the underlying mechanisms. OGD for 3 h followed by reoxygenation for 12 h significantly enhanced the release of pro-inflammatory cytokines and expressions of inflammation-related enzymes in BV-2 cells, which was inhibited by pretreatment with SEI. To elucidate the mechanisms, we studied its effect on upstream signaling pathways. It was found that SEI suppressed the activation of NE-kappa B pathway induced by OGD/R and the MAPK pathway was shown not to be involved. Furthermore, SEI significantly down-regulated TLR4/MyD88 pathway with specifically improving inducible Hsp70 level through increasing HSF-1/DNA binding activity, and these regulations responsive to SEI were attenuated by transfecting Hsp70 siRNA and HSF-1 decoy ODNs. Additionally, SEI exerted similar influence on Hsp70/TLR4/NF-kappa B pathway in rat primary microglial cells. The results suggested that SEI had a potent effect against stroke-induced neuroinflammation through suppressing the TLR4/NE-kappa B pathway by up-regulating Hsp70 dependent on HSF-1. (C) 2016 Elsevier B.V. All rights reserved.
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