期刊
BRAIN RESEARCH
卷 1650, 期 -, 页码 178-183出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2016.09.012
关键词
Hypoxia; Arterial hypertension; Sympathetic nervous system; Astrogliosis
资金
- British Heart Foundation Intermediate Basic Research Science Fellowship for Nephtali Marina [FS/13/5/29927]
- Multiple Sclerosis Society UK [979/12]
- National Multiple Sclerosis Society USA [RG 5056A4]
- Rosetrees Trust, United Kingdom [A997]
- British Heart Foundation [FS/13/5/29927] Funding Source: researchfish
- Rosetrees Trust [M507] Funding Source: researchfish
Increased activity of the sympathetic nervous system has been highlighted as a key factor that contributes to the development and maintenance of arterial hypertension. However, the factors that precipitate sustained increases in sympathetic activity remain poorly understood. Resting tissue oxygen partial pressure (PtO2) in the brainstem of anesthetized spontaneously hypertensive rats (SHRs) has been shown to be lower than in normotensive rats despite normal levels of arterial PO2. A hypoxic environment in the brainstem has been postulated to activate astroglial signalling mechanisms in the rostral ventrolateral medulla (RVLM) which in turn increase the excitability of presympathetic neuronal networks. In this study, we assessed the expression of indirect markers of tissue hypoxia and astroglial cell activation in the RVLM of SHRs and age-matched normotensive Wistar rats. Immunohistochemical labelling for hypoxia-induced factor-1 alpha (HIF-1 alpha) and bound pimonidazole adducts revealed the presence of tissue hypoxia in the RVLM of SHRs. Double immunostaining showed co-localization of bound pimonidazole labelling in putative presympathetic Cl neurons and in astroglial cells. Quantification of glial fibrillary acidic protein (GFAP) immunofluorescence showed relatively higher number of astrocytes and increased GFAP mean grey value density, whilst semi-quantitative analysis of skeletonized GFAP-immunoreactive processes revealed greater % area covered by astrocytic processes in the RVLM of adult SHRs. In conclusion, the morphological findings of tissue hypoxia and astrogliosis within brainstem presympathetic neuronal networks in the SHR support previous observations, showing that low brain stem PtO2 and increased astroglial signalling in the RVLM play an important role in pathological sympathoexcitation associated with the development of arterial hypertension. (C) 2016 The Authors. Published by Elsevier B.V.
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