期刊
DEVELOPMENTAL DYNAMICS
卷 249, 期 6, 页码 711-722出版社
WILEY
DOI: 10.1002/dvdy.158
关键词
cellular dynamics; cruciate ligament; embryonic development; lineage tracing; patella; patellar tendon
资金
- European Molecular Biology Organization
- Fundamental Research Funds for the Central Universities of Central South University
- Stiftelsen Frimurare Barnhuset i Stockholm
- Vetenskapsradet [2016-02835, 2018-02713]
- Chinese Scholarship Council
- Russian Foundation for Basic Research
- Karolinska Institute
- Swedish Research Council [2016-02835, 2018-02713] Funding Source: Swedish Research Council
- Vinnova [2018-02713] Funding Source: Vinnova
Background Cruciate ligament (CL) and patellar tendon (PT) are important elements of the knee joint, uniting femur, patella, and tibia into a single functional unit. So far, knowledge on the developmental mechanism of CL, PT, and patella falls far behind other skeletal tissues. Results Here, employing various lineage tracing strategies we investigate the cellular sources and dynamics that drive CL, PT, and patella formation during mouse embryonic development. We show that Gdf5 and Gli1 are generally expressed in the same cell population that only contributes to CL, but not PT or patella development. In addition, Col2 is expressed in two independent cell populations before and after joint cavitation, where the former contributes to the CL and the dorsal part of the PT and the latter contributes to the patella. Moreover, Prrx1 is always expressed in CL and PT progenitors, but not patella progenitors where it is switched off after joint cavitation. Finally, we reveal that patella development employs different cellular dynamics before and after joint cavitation. Conclusions Our findings delineate the expression changes of several skeletogenesis-related genes before and after joint cavitation, and provide an indication on the cellular dynamics underlying ligament, tendon, and sesamoid bone formation during embryogenesis.
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