期刊
DEVELOPMENTAL CELL
卷 52, 期 2, 页码 210-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2019.12.011
关键词
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资金
- Medical Research Council UK (MRC) [MC_UU_00012/5]
- Human Frontier Science Program [RGY 66/2013]
- European Research Council [311637-MorphoCorDiv]
- Cancer Research UK
- Canadian Institute of Health Research [MOP-142374]
- FRQS Senior investigator career award
- BBSRC ALERT16 equipment grant [BB/R000042]
- BBSRC [BB/R000042/1] Funding Source: UKRI
- MRC [MC_UU_12018/5, MC_UU_00012/5, MR/K015826/1] Funding Source: UKRI
Most metazoan cells entering mitosis undergo characteristic rounding, which is important for accurate spindle positioning and chromosome separation. Rounding is driven by contractile tension generated by myosin motors in the sub-membranous actin cortex. Recent studies highlight that alongside myosin activity, cortical actin organization is a key regulator of cortex tension. Yet, how mitotic actin organization is controlled remains poorly understood. To address this, we characterized the F-actin interactome in spread interphase and round mitotic cells. Using super-resolution microscopy, we then screened for regulators of cortex architecture and identified the intermediate filament vimentin and the actin-vimentin linker plectin as unexpected candidates. We found that vimentin is recruited to the mitotic cortex in a plectin-dependent manner. We then showed that cortical vimentin controls actin network organization and mechanics in mitosis and is required for successful cell division in confinement. Together, our study highlights crucial interactions between cytoskeletal networks during cell division.
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