期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 104, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2019.103566
关键词
Immunoglobulin light chains; Variable families; Oncorhynchus mykiss; Rainbow trout; TNP-KLH
资金
- National Science Foundation [1149553]
- Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2012-01310]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1149553] Funding Source: National Science Foundation
This study characterizes immunoglobulin light chain (IgL) expression and variable family usage in rainbow trout. IgL transcripts were generated by 5' RACE from both immune and TNP-KLH immunized fish. Phylogenetic analysis revealed that the IgL variable regions clustered into seven different families: three kappa families (two newly described in this study), three sigma families, and a single lambda family. IgL1 and IgL3 transcripts expressing identical variable regions were identified and genomic analysis revealed that the two isotypes are colocalized on chromosomes 7, 15, 18, and 21 allowing for potential rearrangement between clusters. Fish were immunized with TNP-KLH (n = 5) and percent expression of IgL1, IgL2, IgL3, and IgL4 measured by qRT-PCR from immune tissues and magnetically sorted TNP-specific lymphocyte populations. In all samples IgL1 constituted 80-95% of the transcripts. The percentage of anti-TNP specific IgL1 transcripts was measured in naive, unsorted, and TNP-specific cell populations of TNP-ICLH fish (n = 3) and found to be significantly higher in the TNP positive cell population (21%) compared to the naive population (1%; p = 0.02) suggesting that there is a selection of TNP specific IgL sequences.
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